General Biology I · Module 13 · DNA structure and replication

DNA Structure and Replication Lab

Run a replication fork and take enzymes away to see what breaks, spin DNA in a density gradient for each model, practise every DNA calculation with animated worked solutions, match enzymes and experiments, and use these ideas in medicine and research. Nothing here is graded.

Part 1: Replication Fork Bench

All enzymes present. Watch the leading strand grow continuously and the lagging strand form Okazaki fragments.
Parent (template) strand New DNA RNA primer SSB Red tick: unsealed nick Graph: leading strand Graph: lagging strand
Live readouts
MeasureValue

Teaching model. The fork moves much slower than a real one, primers are drawn about ten times longer than real (about 10 nucleotides), and enzymes are drawn huge. Counts and lengths are converted to real nucleotides and real seconds using about 1000 nucleotides per second per fork in bacteria and about 50 in eukaryotes.