Eukaryotic Gene Regulation Lab

Part 1: Enhancer Lab

Enhancer lab readouts
Measure	Value

Mode

1 · Pick an element

2 · Place it in a slot

Slots −3 to −1 are upstream of the promoter; +1 and +2 are downstream of the gene. You can also tap a slot in the picture.

Transcription factors

Activator (binds enhancers) Repressor (binds silencers)

Chromatin modifiers at the promoter

Tethered HAT (acetylation) Tethered HDAC (deacetylation) Tethered PRC2 (H3K27me3) Tethered SUV39H1 (H3K9me3, HP1) DNMT3: methylate the promoter CpG island Add an HDAC inhibitor drug

Eukaryotic Gene Regulation Lab

Part 1: Enhancer Lab

Part 2: Problem Trainer

Part 3: Sort the Controls

Part 4: Regulation in the Real World